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Simultaneous determination of acetaminophen (paracetamol) and ascorbic acid in pharmaceutical formulations by LC coupled to a screen printed carbon based amperometric detector.

par Kauffmann, Jean-Michel ;Gokce, G.;Sidali, T.;Aydogmus, Zeynep;Sarakbi, Ahmad
Référence Electroanalysis, 23, page (980-987)
Publication Publié, 2011

Article révisé par les pairs

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Résumé :

The assay of acetaminophen (APAP) and ascorbic acid (AA) was performed by liquid chromatography (LC) with amperometric detection at a screen printed carbon based electrode poised at +0.6 V versus Ag pseudoreference electrode. A microparticle based conventional RP-C18 and a monolithic RP-C18 column were studied comparatively for the analytes separation using a mobile phase based on 0. 1 M phosphate buffer-methanol 91:9 (v/v) at pH 6.5. The linear calibration range for both compounds was comprised within 2×10-7-1×10-4 M. Limit of detection (LOD) for APAP and AA was 2×10-7 and 5×10-7 M, respectively and the limit of quantification (LOQ) was 5. 10-7and 1×10-6 M, respectively. Square wave voltammetry at a Nafion modified glassy carbon electrode was developed and applied to the determination of APAP in order to validate the results obtained by the monolithic LC method for drug formulations. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.