par Portetelle, Daniel 
;Bruck, Claudine ;Mammerickx, Marc;Burny, Arsène
Référence Journal of virological methods, 6, 1, page (19-29)
Publication Publié, 1983
;Bruck, Claudine ;Mammerickx, Marc;Burny, Arsène Référence Journal of virological methods, 6, 1, page (19-29)
Publication Publié, 1983
Article révisé par les pairs
| Résumé : | A variant of the ELISA technique, involving a monoclonal anti-gp51 antibody yields a highly sesnsitive method for detection of borne leukaemia virus (BLV) antibodies. The gp51 antigen coated microtitre plates are obtained by incubation of plastic-absorbed monoclonal antibodies with a non-purified mixture of BLV antigens. Sera to be tested are incubated in the wells of the gp51-coated plates and bound antibodies are revealed by an enzyme-linked antibovine immunoglobulin reagent. This test is as sensitive as liquid phase radioimmunoassay using the same gp51 antigen and thus appears as a highly sensitive, practical, rapid and cheap method for the detection of BLV antibodies. |
