Résumé : In this paper the effect of noise on both perceptual and automatic evaluation of the glottal cycle length in irregular voice signals (sustained vowels) is studied. The reliability of four tools for voice analysis (MDVP, Praat, AMPEX, and BioVoice) is compared to visual inspection made by trained clinicians using two measures of voice signal irregularity: the jitter (J) and the coefficient of variation of the fundamental frequency (F0CV). The purpose is also to test to what extent of irregularity trained raters are capable of determining visually the glottal cycle length as compared to dedicated software tools. For a perfect control of the amount of jitter and noise put in, data consist of synthesized sustained vowels corrupted by increasing jitter and noise. Both jitter and noise can be varied to the desired extent according to built-in functions. All the tools give almost reliable measurements up to 15% of jitter, for low or moderate noise, while only few of them are reliable for higher jitter and noise levels and would thus be suited for perturbation measures in strongly irregular voice signals. As shown in Part I of this work, for low noise levels the results obtained by visual inspection from expert raters are comparable or better than those obtained with the tools presented here, at the expense of a larger amount of time devoted to searching visually for the glottal cycle lengths in the signal waveform. In this paper it is shown that results rapidly deteriorate with increasing noise. Hence, the use of a robust tool for voice analysis can give valid support to clinicians in term of reliability, reproducibility of results, and time-saving. © 2011 Informa UK, Ltd.