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Analysis of the peripheral T-cell repertoire in kidney transplant patients

par Miqueu, P.;Deguauque, N.;Guillet, M.;Giral, M.;Ruíz, Catherine L.;Pallier, A.;Braudeau, C.;Roussey-Kesler, G.;Ashton-Chess, J.;Doré, JC;Thervet, E.;Legendre, Christophe;Hernandez-Fuentes, Maria P;Warrens, AN;Goldman, Michel ;Volk, HD;Janssen, U.;Wood, Kathryn;Lechler, RI;Bertrand, Dominique;Sebille, V.;Soulillou, JP;Brouard, S.
Référence European Journal of Immunology, 40, 11, page (3280-3290)
Publication Publié, 2010

Article révisé par les pairs

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Résumé :

The long-term stability of renal grafts depends on the absence of chronic rejection. As T cells play a key role in rejection processes, analyzing the T-cell repertoire may be useful for understanding graft function outcomes. We have therefore investigated the power of a new statistical tool, used to analyze the peripheral blood TCR repertoire, for determining immunological differences in a group of 229 stable renal transplant patients undergoing immunosuppression. Despite selecting the patients according to stringent criteria, the patients displayed heterogeneous T-cell repertoire usage, ranging from unbiased to highly selected TCR repertoires; a skewed TCR repertoire correlating with an increase in the CD8 +/CD4 + T-cell ratio. T-cell repertoire patterns were compared in patients with clinically opposing outcomes i.e. stable drug-free operationally tolerant recipients and patients with the "suspicious" form of humoral chronic rejection and were found significantly different, from polyclonal to highly selected TCR repertoires, respectively. Moreover, a selected TCR repertoire was found to positively correlate with the Banff score grade. Collectively, these data suggest that TCR repertoire categorization might be included in the calculation of a composite score for the follow-up of patients after kidney transplantation. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.