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Monitoring of cellular responses after vaccination against tetanus toxoid: comparison of the measurement of IFN-gamma production by ELISA, ELISPOT, flow cytometry and real-time PCR.

par Tassignon, Joël ;Burny, Wivine ;Dahmani, Samira;Zhou, Ling ;Stordeur, Patrick ;Byl, Baudouin ;De Groote, Donat
Référence Journal of immunological methods, 305, 2, page (188-198)
Publication Publié, 2005-10

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Titre:	Monitoring of cellular responses after vaccination against tetanus toxoid: comparison of the measurement of IFN-gamma production by ELISA, ELISPOT, flow cytometry and real-time PCR.
Auteur:	Tassignon, Joël; Burny, Wivine; Dahmani, Samira; Zhou, Ling; Stordeur, Patrick; Byl, Baudouin; De Groote, Donat
Informations sur la publication:	Journal of immunological methods, 305, 2, page (188-198)
Statut de publication:	Publié, 2005-10
Sujet CREF:	Médecine pathologie humaine
	Sciences biomédicales
MeSH keywords:	Adult
	CD4-Positive T-Lymphocytes -- immunology
	Enzyme-Linked Immunosorbent Assay
	Female
	Flow Cytometry
	Humans
	Immunologic Memory
	Interferon-gamma -- biosynthesis -- blood -- genetics -- immunology
	Male
	Middle Aged
	Monitoring, Immunologic -- methods
	RNA, Messenger -- chemistry -- genetics
	Reverse Transcriptase Polymerase Chain Reaction
	Sensitivity and Specificity
	Statistics, Nonparametric
	Tetanus Toxoid -- immunology
	Vaccination
Note générale:	Comparative Study
	Journal Article
Langue:	Anglais
Identificateurs:	urn:issn:0022-1759
	info:doi/10.1016/j.jim.2005.07.014
	info:pii/S0022-1759(05)00234-6
	info:pmid/16157348