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Real-time PCR for determining capsular serotypes of Haemophilus influenzae.

par Maaroufi, Younes ;De Bruyne, Jean Marc;Heymans, Corine;Crokaert, Françoise
Référence Journal of clinical microbiology, 45, 7, page (2305-2308)
Publication Publié, 2007-07

Article révisé par les pairs

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Résumé :

A two-step real-time PCR assay targeting all six capsulation loci of Haemophilus influenzae (i.e., serotypes a to f) was developed and compared with a previously published qualitative PCR assay by using 131 H. influenzae clinical isolates. There was a 98.5% concordance between the two tests. The sensitivity of detection of capsular type-specific reference strains of H. influenzae a to c (10(1) CFU/PCR) was higher than that for type e (10(3) CFU/PCR) and types d and f (10(4) CFU/PCR), and a broader dynamic range was obtained (5 to 8 log(10) units). No cross-reaction was observed with bacteria commonly isolated from the respiratory tract. We showed that both PCR assays are more reliable than slide agglutination serotyping. The real-time PCR-based assay seems to be an alternative of choice for the epidemiological follow-up of H. influenzae invasive infections.