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Isocratic high-performance liquid chromatographic method for the separation of isradipine and its main metabolites Application to in vitro metabolization by h3A4/OR cells

par Bidouil, Sylviane ;Dubois, Jacques ;Hanocq, Michel
Référence Journal of chromatography. B, Biomedical applications, 693, 2, page (359-366)
Publication Publié, 1997-06

Article révisé par les pairs

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Résumé :

A reversed-phase HPLC method was developed for the study of isradipine oxidation in vitro. The drug and its main metabolites were determined after extraction from culture media and from h3A4/OR cells having the cytochrome P450 isoenzyme involved in the xenobiotic metabolization. The HPLC assay was fully validated and was used to follow the biotransformation kinetics of isradipine.