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Expansion of regulatory CD8+ CD25+ T cells after neonatal alloimmunization

par Adams, Brigitte ;Dubois, Aurore ;Delbauve, Sandrine ;Debock, Isabelle ;Lhommé, Frédéric ;Goldman, Michel ;Flamand, Véronique
Référence Clinical and experimental immunology, 163, 3, page (354-361)
Publication Publié, 2011-03

Article révisé par les pairs

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Résumé :

Transplantation tolerance induced by neonatal injection of semi-allogeneic spleen cells is associated with a pathological syndrome caused by T helper type 2 (Th2) differentiation of donor-specific CD4+ T lymphocytes. We have shown previously that this Th2-biased response is inhibited by host CD8+ T cells. Herein, we demonstrate that upon neonatal immunization with (A/J×BALB/c)F1 spleen cells, BALB/c mice expand a population of CD8+ T cells expressing both CD25 and forkhead box P3 (FoxP3) markers. In this setting, CD8+CD25+ T cells predominantly produce interferon (IFN)-γ and interleukin (IL)-10 and are efficient in controlling IL-4, IL-5 and IL-13 production by donor-specific CD4+ T cells in vitro. CD8+FoxP3- T cells are single producers of IFN-γ or IL-10, whereas CD8+FoxP3+ T cells are double producers of IFN-γ and IL-10. We further demonstrate that IFN-γ and IL-10 are two major cytokines produced by CD8+ T cells involved in the in vivo regulation of Th2-type pathology. In this setting, we conclude that neonatal alloimmunization induces the expansion of several regulatory CD8+ T cells which may control Th2 activities via IFN-γ and IL-10. © 2010 The Authors. Clinical and Experimental Immunology © 2010 British Society for Immunology.