Anti DNA antibodies are not restricted to a specific pattern of fluorescence on HEp2 cells

Servais, Geneviève; Karmali, Rafik; Guillaume, Marie-Paule; Badot, Valérie; Duchateau, Jean; Corazza, Francis

doi:doi/10.1515/CCLM.2009.122

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Anti DNA antibodies are not restricted to a specific pattern of fluorescence on HEp2 cells

par Servais, Geneviève ;Karmali, Rafik

;Guillaume, Marie-Paule

;Badot, Valérie

;Duchateau, Jean

;Corazza, Francis

Référence Clinical chemistry and laboratory medicine, 47, 5, page (543-549)
Publication Publié, 2009-05

Article révisé par les pairs

Résumé :

Antinuclear autoantibody determination relies on an initial screening step using immunofluorescence on HEp2 cells. This may be followed by anti-deoxyribonucleic acid (DNA) determination, if the fluorescence of nuclei is homogeneous. We assessed the validity of a restricted algorithm and compared this to a more comprehensive algorithm that accepted any nuclear pattern as a positive indicator.

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