Résumé : Objective: To assess the adequacy of a multidisciplinary approach providing information to couples affected by HIV before ART. Design: Prospective observational study. Setting: RT centre and infectious disease clinic, public university hospital. Patients: 50 couples with at least one HIV-infected partner. Interventions: Multidisciplinary approach towards ART by various intervening physicians (specialist in fertility, infectious diseases, paediatrics, obstetrics, psychiatry). Main outcome measured: We analysed specifically the cases in which the staff did not accept and the patient's compliance to the counselling procedures. Results: Among the 150 couples, 30 did not complete the procedure and were lost to follow-up. The remaining 120 couples were evaluated: 89 couples were accepted, 5 were temporarily refused and 26 were refused definitively. The major reasons for refusal were medical reasons (n = 13). Conclusion: Because of the high refusal rate and the drop of rate, a multidisciplinary approach is mandatory before initiating ART in seropositive couples. © 2009 Elsevier Ireland Ltd. All rights reserved.