Résumé : A capillary gas chromatographic method has been developed for the determination of canthinone alkaloids. The method was applied to the semi-quantitative determination of canthin-6-one, 1-methoxycanthin-6-one, 1-hydroxycanthin-6-one, 8-hydroxycanthin-6-one, 10-hydroxycanthin-6-one, and canthin-6-one-3-N-oxide in callus and shoot cultures, and in root barks of Hannoa chlorantha. The results showed that their concentrations were highly dependent on the sample origin and on the culture conditions.