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Glucagon and insulin release by the in vitro perfused rat pancreas. Influence of the colloid composition of the perfusate

par Leclercq Meyer, Viviane ;Marchand, Jocelyne;Leclercq, Raoul ;Malaisse, Willy
Référence Diabète & métabolisme, 2, 2, page (57-65)
Publication Publié, 1976

Article révisé par les pairs

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Résumé :

The rate of insulin and glucagon secretion by the in vitro perfused rat pancreas greatly varied according to the type, commercial origin and lot of colloid added to the perfusate. Such a variability was evidenced in control experiments performed throughout at a given glucose concentration (3.3 or 16.6 mM), as well as in perfusions including a stimulus (calcium deprivation, or a rise in glucose concentration from 3.3 to 8.3 mM). No correlation could be established between the rate of hormonal secretion and any of the parameters of the perfusion system. No interference with the radioimmunoassay was noticed. These findings suggest that unidentified factor(s) present in some albumin preparations may affect the secretory responsiveness of the endocrine pancreas. Because adequate and reproductive secretory behaviour of both the alpha and beta cell was consistently recorded with different lots of dextran, media containing dextran and a small amount of albumin (0.5%, w/v) are recommended for use in the in vitro perfused rat pancreas.