Titre:
  • A simple and rapid protocol to non-enzymatically dissociate fresh human tissues for the analysis of infiltrating lymphocytes
Auteur:Garaud, Soizic; Gu-Trantien, Chunyan; Lodewyckx, Jean Nicolas; Boisson, Anaïs; De Silva, Pushpamali; Buisseret, Laurence; Migliori, Edoardo; Libin, Myriam; Naveaux, Céline; Duvillier, Hugues; Willard-Gallo, Karen
Informations sur la publication:Journal of Visualized Experiments, 94, e52392
Statut de publication:Publié, 2014-12
Sujet CREF:Urbanisme et architecture [génie civil]
Bâtiments génie civil transports
Neurosciences cognitives
Génie chimique
Microbiologie et protistologie [bacteriol.,virolog.,mycolog.]
Immunologie
Sciences bio-médicales et agricoles
Mots-clés:Breast cancer
CD45+
Fresh tissue homogenate
Immunology
Issue 94
Non-enzymatic dissociation
Primary tissue supernatant
Tumor immunology
Tumor infiltrating lymphocytes
MeSH keywords:Antigens, CD45 -- chemistry
Breast -- cytology -- immunology
Breast Neoplasms -- immunology -- pathology
Cytological Techniques -- methods
Female
Humans
Lymphocytes, Tumor-Infiltrating -- cytology -- immunology -- pathology
Note générale:SCOPUS: ar.j
SCOPUS: ar.j
Langue:Anglais
Identificateurs:urn:issn:1940-087X
info:doi/10.3791/52392
info:scp/84917695112
info:pmid/25548995