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Reduced glutamate decarboxylase activity in rat islet β cells which survived streptozotocin-induced cytotoxicity

par Ling, Zhidong;Pipeleers, Daniel;Malaisse Lagae, Francine ;Malaisse, Willy
Référence FEBS letters, 324, 3, page (262-264)
Publication Publié, 1993-06

Article révisé par les pairs

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Résumé :

Rat pancreatic β cells exhibit a 16-fold higher glutamate decarboxylase (GAD) activity than islet non-β cells, but a similar glutamate dehydrogenase (GDH) activity, β Cells which survive exposure to 2 mM streptozotocin only contain 10 percent of the GAD activity of control cells, but their GDH activity remains unaltered. Culture of streptozotocin-treated β cell preparations with 2 mM nicotinamide reduces the number of dead cells and prevents in part the decline in GAD activity of surviving β cells. These data indicate that loss in activity of the β cell specific enzyme GAD can serve as marker for β cells which survived a destructive process. It is furthermore demonstrated that nicotinamide increases the percent surviving cells and decreases their loss in GAD activity. © 1993.