par Vandevoorde, Aurélie;Ascenzo, Sabrina;Miendje Deyi, Yvette Véro ;Mascart, Georges;Mansbach, Anne-Laure ;Landsberg, Marguerite ;Drèze, Pierre-Alexandre ;Steer, Andrew C;Van Melderen, Laurence ;Smeesters, Pierre
Référence The Pediatric infectious disease journal
Publication Publié, 2012-12
Référence The Pediatric infectious disease journal
Publication Publié, 2012-12
Article révisé par les pairs
Résumé : | This study describes for the first time heterogeneity of antibiotic resistance profiles among group A Streptococcus (GAS) isolates originating from a single throat swab in patients with acute pharyngitis. For each throat swab, ten GAS colonies were randomly selected from the primary plate and subcultured to a secondary plate. These isolates were characterized by various phenotypic and genotypic methods. Our results demonstrated that differing antibiotic resistance profiles were present in 19% of pediatric patients with acute pharyngitis before antimicrobial treatment. This heterogeneity likely resulted from horizontal gene transfer amongst streptococcal isolates sharing the same genetic background. As only a minority of colonies displayed antibiotic resistance among these heterogeneous samples, a classical diagnostic antibiogram would have classified them in most instances as 'susceptible' although therapeutic failure could be caused by the proliferation of resistant strains after initiation of antibiotic treatment. |