par Charles, Catherine 
;Ouedraogo, Malika ;Belayew, Alexandra ;Duez, Pierre
Référence Analytical biochemistry, 425, page (76-79)
Publication Publié, 2012
;Ouedraogo, Malika ;Belayew, Alexandra ;Duez, Pierre Référence Analytical biochemistry, 425, page (76-79)
Publication Publié, 2012
Article révisé par les pairs
| Résumé : | Oligomerization of linearized plasmids by nuclear proteins extracts, a recognized measure of nonhomologous end-joining (NHEJ) repair capacity, is typically assessed through agarose gel electrophoresis, a labor-intensive procedure. In the current study, a more convenient NHEJ assay was developed using microchips that allow scaled-down separation and quantification. This microchip method allows a considerable reduction in sample amount and analysis time with similar costs and comparable or slightly better precision. Data obtained with quercetin and wortmannin show that the method can be applied to the screening of food components and natural products for positive and negative modulators of NHEJ, potential chemopreventive and indirect genotoxic compounds, respectively. © 2012 Elsevier Inc. All rights reserved. |
