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The monovalent anions chloride and azide as potent activators of NaF- and p(NH)ppG-stimulation of pancreatic adenylate cyclase

par Svoboda, Michal ;Christophe, Jean
Référence FEBS letters, 86, 2, page (230-234)
Publication Publié, 1978

Article révisé par les pairs

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Résumé :

NaCl and NaN3 used at maximally effective concentration were found to activate the adenylate cyclase of rat pancreatic plasma membranes 1.3-2.0-fold under basal conditions, and under stimulation by secretin, the C-terminal octapeptide of cholecystokinin-pancreozymin (OC-PZ) and GTP, or by each peptide offered in combination with GTP or p(NH)ppG. Stimulation of adenylate cyclase by maximally effective concentrations of p(NH)ppG alone or by NaF was enhanced by NaCl or NaN3 to a greater degree than was observed in the above-mentioned cases. The 5.1-fold anionic activation of p(NH)ppG-stimulated adenylate cyclase was due to an increase in V(max). The 3.4-fold anionic activation of NaF stimulation resulted from an increase in app. V(max) and a lower app. K(m). It is concluded that the amplitude of the activating effects of the monovalent anions Cl- and N3- are highly dependent on the configuration of the adenylate cyclase system.