par Roosens, Nancy 
;Bernard, Catherine ;Leplae, Raphaël ;Verbruggen, Nathalie
Référence Zeitschrift für Naturforschung. C, Journal of biosciences, 60, 3-4, page (224-228)
Publication Publié, 2005
;Bernard, Catherine ;Leplae, Raphaël ;Verbruggen, Nathalie Référence Zeitschrift für Naturforschung. C, Journal of biosciences, 60, 3-4, page (224-228)
Publication Publié, 2005
Article révisé par les pairs
| Résumé : | A functional screening in yeast allowed to identify various cDNAs from the Cd/Zn hyperaccumulator Thlaspi caerulescens. TcMT3 displayed high identity with its closest homologue in Arabidopsis thaliana but variation in its Cys residues. Functional analysis in yeast supported a higher binding capacity for Cu, but not for Cd or Zn, of TcMT3 compared to AtMT3. Expression analysis in plants indicated that metallothionein 3 (MT3) like all the other T. caerulescens genes from the screen studied is overexpressed in all studied populations of T. caerulescens compared to A. thaliana. TcMT3 was induced by Cu, but not by Cd. Moreover significant variation in expression within T. caerulescens populations that have contrasting tolerance and accumulation capacities indicated a possible local adaptation of MT3. © 2005 Verlag der Zeitschrift für Naturforschung. |
