• Citer

IL-12 prevents neonatal induction of transplantation tolerance in mice.

par Donckier De Donceel, Vincent ;Flamand, Véronique ;Desalle, Fabrice ;Vanderhaeghen, Marie-Line;de Veerman, Marijke;Thielemans, Kris;Abramowicz, Daniel ;Goldman, Michel
Référence European Journal of Immunology, 28, 4, page (1426-1430)
Publication Publié, 1998-04

Article révisé par les pairs

• Accès en ligne
• Détails
• Contenu
• Statistiques

Résumé :

We investigated the effect of IL-12 on the induction of transplantation tolerance by neonatal injection of allogenic cells. We first observed that injection of newborn BALB/c mice with IL-12 and (A/J x BALB/c)F1 spleen cells prevented the Th2 alloimmune response induced by neonatal inoculation of F1 cells alone and allowed the differentiation of T cells secreting high amounts of IL-2 and IFN-gamma in mixed lymphocyte cultures with donor-type stimulators. Furthermore, IL-12 administration resulted in the emergence of anti-donor cytotoxic T lymphocyte responses although at lower levels than in control uninjected mice. In parallel, we found that mice injected at birth with IL-12 and F1 cells did not develop chimerism and were able to reject a donor-type skin graft as efficiently as control mice. We conclude that IL-12 inhibits the Th2 polarization of the newborn response to alloantigens and prevents thereby the establishment of transplantation tolerance.