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Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress.

par Guo, Dehuang;Han, Junyan;Adam, Bao-Ling;Colburn, Nancy H;Wang, Mong-Heng;Dong, Zheng;Eizirik, Decio L. ;She, Jin-Xiong;Wang, Cong-Yi
Référence Biochemical and biophysical research communications, 337, 4, page (1308-1318)
Publication Publié, 2005

Article révisé par les pairs

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Résumé :

The substrates of SUMO4, a novel member for the SUMO gene family, were characterized in HEK293 cells cultured under serum starvation by proteomic analysis. We identified 90 SUMO4 substrates including anti-stress proteins such as antioxidant enzymes and molecular chaperones or co-chaperones. The substrates also include proteins involved in the regulation of DNA repair and synthesis, RNA processing, protein degradation, and glucose metabolism. Several SUMO4-associated transcription factors were characterized by Western blot analyses. AP-1 was selected for in vitro conjugation assays to confirm SUMO4 sumoylation of these transcription factors. Further functional analyses of the transcription factors suggested that SUMO4 sumoylation represses AP-1 and AP-2alpha transcriptional activity, but enhances GR DNA binding capacity. These results demonstrate that SUMO4 sumoylation may play an important role in the regulation of intracellular stress.