• Citer

Human serum lyses Trypanosoma brucei by triggering uncontrolled swelling of the parasite lysosome.

par Vanhollebeke, Benoît ;Lecordier, Laurence ;Perez-Morga, David ;Amiguet Vercher, Amélia ;Pays, Etienne
Référence The Journal of eukaryotic microbiology, 54, 5, page (448-451)
Publication Publié, 2007

Article révisé par les pairs

• Accès en ligne
• Détails
• Contenu
• Statistiques

Résumé :

Trypanosoma brucei brucei infects a wide range of mammals, but is unable to infect humans because this subspecies is lysed by normal human serum (NHS). The phenotype of cellular lysis is debated. For some authors the lysosome undergoes osmotic swelling due to massive influx of chloride ions from the cytoplasmic compartment, but others describe multiple small cytoplasmic vacuoles and general swelling of the cellular body. Using population-level imaging of live immobilized trypanosomes throughout the lysis process, we report that specific swelling of the lysosome is a genuine and major characteristic of NHS-mediated lysis and that this phenotype is independent of the strain of trypanosomes and of NHS aging or damaging. Thus, irrespective of experimental conditions NHS reproducibly induced the swelling of the parasite lysosome.