par López Mora, Néstor;Bahreman, Azadeh;Valkenier, Hennie ;Li, Hongyu;Sharp, Thomas H.;Sheppard, David N.;Davis, Anthony P.;Kros, Alexander
Référence Chemical science, 7, page (1768-1772)
Publication Publié, 2016-01-07
Référence Chemical science, 7, page (1768-1772)
Publication Publié, 2016-01-07
Article révisé par les pairs
Résumé : | Synthetic anion transporters (anionophores) have potential as biomedical research tools and therapeutics. However, the efficient and specific delivery of these highly lipophilic molecules to a target cell membrane is non-trivial. Here, we investigate the delivery of a powerful anionophore to artificial and cell membranes using a coiled-coil-based delivery system inspired by SNARE membrane fusion proteins. Incorporation of complementary lipopeptides into the lipid membranes of liposomes and cell-sized giant unilamellar vesicles (GUVs) facilitated the delivery of a powerful anionophore into GUVs, where its anion transport activity was monitored in real time by fluorescence microscopy. Similar results were achieved using live cells engineered to express a halide-sensitive fluorophore. We conclude that coiled-coil driven membrane fusion is a highly efficient system to deliver anionophores to target cell membranes. |