Résumé : This study deals with the development of an original epi-fluorescence-based protocol to evaluate the anti-biofouling character of atmospheric pressure plasma-polymerized poly(ethylene glycol) samples. In this work, fluorescence microscopy is considered as an alternative to XPS for the evaluation of protein adsorption. The full set of samples is tested before and after bovine serum albumin-fluorescein isothiocyanate treatment. It appears that their anti-biofouling property, expressed by the variation of fluorescence intensity, is reduced when the power of the discharge is increased. The reproducibility of the experiments and the relatively high sensitivity of the proposed method are underlined.