par Verschueren, Hendrik;Van Hecke, Dominique;Pokorni-Hannecart, Eleonora 
;Dekegel, Daniel ;De Baetselier, Patrick
Référence Clinical & experimental metastasis, 7, 5, page (541-555)
Publication Publié, 1989-09
;Dekegel, Daniel ;De Baetselier, PatrickRéférence Clinical & experimental metastasis, 7, 5, page (541-555)
Publication Publié, 1989-09
Article révisé par les pairs
| Résumé : | A simple monolayer invasion assay (MIA) was recently developed using confluent fibroblastic cells as a target and variants of the BW5147 murine T-cell lymphoma as invading cells. Metastatic variants were consistently invasive in the MIA whereas non-metastatic cells were not. In this paper it is reported that pertussis toxin (PT) treatment of a highly metastatic and invasive variant caused a marked delay of invasion in the MIA at concentrations from 50 pg/ml upwards. Surprisingly, PT treatment of the non-metastatic, non-invasive parental BW5147 cells induced a moderate but significant level of invasion. Morphometric analysis showed that PT provoked an increased pseudopodal activity in cells in which it also caused increased invasive potential, and a decreased motility in cells with decreased invasiveness. This finding strengthens the perception that invasive potential and the capability to perform shape changes are related characteristics in these lymphoma cells. © 1989 Taylor & Francis Ltd. |
