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Effect of subinhibitory concentrations of ketoconazole on Candida albicans measured by radiometric method

par Yourassowsky, Eugène ;Schoonjans, J.;Gaeng, F.
Référence Current therapeutic research, 40, 5, page (965-968)
Publication Publié, 1986

Article révisé par les pairs

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Résumé :

The in vitro measurement of antifungal drug activity is subject to controversy and the culture medium is particularly controversial. By microtiter plate technique the minimum inhibitory concentrations (MICs) of ketoconazole on Candida albicans were similar in brain heart infusion broth and tryptic soy broth. This observation allowed the use of tryptic soy broth vials manufactured for radiometric method by Bactec. A growth curve performed during a 14-day period was obtained by measuring the 14CO2 with the Bactec. With five different strains, a new vial was used each day. Reduced growth rate was found in concentrations of ketoconazole 100 times lower than the MIC. These data may be considered as a minimum antibiotic concentration (MAC). The clinical implication of the MAC values may be interesting to establish a good in vitro/in vivo correlation.