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Chromatographic separation of isoenzymes of creatine phosphokinase from normal serum after myocardial infarction. Study of conditions of estimation and new technique of demonstration of the three isoenzymes using a LKB Reaction Analyzer 8600 (French)

par Leunis, Jean-Claude J.
Référence Annales de biologie clinique, 31, 5, page (369-375)
Publication Publié, 1973

Article révisé par les pairs

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Résumé :

The 3 isoenzymes of creatine phosphokinase (CPK) were isolated by chromatography on DE 52 from normal serum and after myocardial injury. After heart infarct the MB form is increased. The Michaelis constants and the optimum pH activity of each isoenzyme were studied. The acidity of reduced glutathione added to the reaction mixture is sufficient to bring the pH value to 6.95 and this increases the sensitivity of the serum CPK determination by about 30%. The determination of the activity at different pH values allows the visualization of the 3 isoenzymes.