par Vincke, Bernard 
;Devleeschouwer, Michel ;Crotteux, Jeanne ;Patriarche, Gaston
Référence International journal of pharmaceutics, 21, 3, page (265-275)
Publication Publié, 1984
;Devleeschouwer, Michel ;Crotteux, Jeanne ;Patriarche, Gaston Référence International journal of pharmaceutics, 21, 3, page (265-275)
Publication Publié, 1984
Article révisé par les pairs
| Résumé : | Two bacterial electrodes have been studied for the determination of nicotinamide (vitamin PP) with a linear range of 2.8 × 10-4 M to 2 × 10 -2 M. The used strains, although taxonomicaly different and differently improved (E. coli mutated and B. pumilus induced), present the same nicotinamide deaminase activity, able to be used for analytical assays. Their long-term stability (more than 100-fold higher than the purified enzyme) is realized by the regeneration of living cells on the electrode itself. The parameters involved in this type of electrode construction are discussed. This type of bacterial electrodes presents a very good selectivity for nicotinamide in multivitamin pharmaceutical formulations. © 1984. |
