par Triplett, Edward E.L.;Lievens, Andrée 
;Baltus, Elyane
Référence Experimental cell research, 38, 2, page (366-378)
Publication Publié, 1965-05
;Baltus, Elyane Référence Experimental cell research, 38, 2, page (366-378)
Publication Publié, 1965-05
Article révisé par les pairs
| Résumé : | Quantitative studies on a family of acid phosphatase isozymes in nucleate and enucleate Acetabularia mediterranea fragments have been performed. A photometric method of measuring enzyme activity visualized as a lead sulfide deposit on agar electrophoretic plates is described. Five isozymes were found; three had activities large enough to quantify. Preliminary studies were made on the specificity of these enzymes. In nucleate fragments these enzymes appear to be correlated in activity with the rate of growth in length of the organism and the appearance of the apical cap. In enucleate fragments there is no increase in total protein after the tenth post-surgical day, but an apical cap may still be formed. Two of the three enzymes measured increased in activity at the time of cap formation in enucleate plants. The third continued to decrease in activity throughout the experiment. The first two enzymes are associated in large part with chloroplasts and mitochondria, the third is not. It is concluded that the rate of increase in activity of the first two enzymes is not limited by the rate of synthesis of m-RNA by the nucleus. © 1965. |
