• Citer

Replication of mini-mu prophage DNA

par Waggoner, Barbara;Pato, Martin Leon;Toussaint, Ariane ;Faelen, Michel
Référence Virology, 113, 1, page (379-387)
Publication Publié, 1981

Article révisé par les pairs

• Accès en ligne
• Détails
• Contenu
• Statistiques

Résumé :

Thermoinducible mini-Mu prophage which have internal deletions spanning various portions of the αG regions were used to analyze the replication process of bacteriophage Mu. Two mini-Mu's, Mu Δ26 and Mu Δ123, containing at least the repressor gene c, the replication genes A and B, and both termini were shown to replicate upon induction even though most of the internal region of the phage DNA was deleted. Mini-Mu (A+B-), deleted for part of the B gene, did not replicate upon induction; complementation with nonreplicating left end Mu fragments supplying B gene product resulted in a very low level of Mu replication, whereas fragments supplying gene B and additional functional DNA between genes B and C allowed extensive replication of the mini-Mu DNA. These results indicate that in the region between genes B and C a function(s) exists which amplifies the replication of Mu DNA. © 1981.