par Koopman, Berend B.J.;Van Der Molen, Jan;Wolthers, E.G. E.G.;Vanderpas, Jean-Baptiste 
Référence Journal of Chromatography B: Biomedical Sciences and Applications, 416, C, page (1-13)
Publication Publié, 1987
Référence Journal of Chromatography B: Biomedical Sciences and Applications, 416, C, page (1-13)
Publication Publié, 1987
Article révisé par les pairs
| Résumé : | The simultaneous determination of some hydroxycholesterols in human serum samples is described. The procedure is based on hydrolysis and extraction of these compounds in serum samples, followed by removal of especially cholesterol (making use of reversed-phase high-performance liquid chromatography) and derivatization of the purified compounds to their trimethylsilyl ethers and subsequent gas chromatography using flame ionization detection. Serum levels of 7α-hydroxycholesterol, 7β-hydroxycholesterol and 26-hydroxycholesterol were determined in several groups of patients: (a) normals, (b) untreated patients suffering from cerebrotendinous xanthomatoais, (c) patients suffering from cerebrotendinous xanthomatosis and treated with either chenodeoxycholic acid or cholic acid in an effective dose, (d) patients suffering from cerebro-hepato-renal syndrome, (e) patients suffering from hypercholesterolemia and treated with cholestyramine for prolonged periods and (f) one patient presumed to be suffering from an inborn error of metabolism in bile acid synthesis. It can be concluded that the 7α-hydyroxycholesterol concentration in serum is a good parameter for establishing disorders involving the metabolic conversion of 7α-hydroxycholesterol towards bile acids. In addition, 26-hydroxycholesterol levels in patients suffering from cerebrotendinous xanthomatosis are beyond detectable limits, even during treatment with bile acids in an effective dose, whereas in all other conditions this compound is substantially present. © 1987. |
