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Phosphorylation in vitro of proteins in the pancreas and parotids of rats: Effects of hormonal secretagogues and cyclic nucleotides

par Lambert, Monique;Camus, Jean Claude ;Christophe, Jean
Référence Biochemical pharmacology, 24, 19, page (1755-1758)
Publication Publié, 1975-10

Article révisé par les pairs

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Résumé :

(1) Caerulein 10-9 M and carbamylcholine 5·10-6 M provoked a 3-fold increase in amylase secretion and a 50% stimulation of protein phosphorylation in rat pancreas fragments. Secretin exerted similar effects, though more moderate, at 5·10-7 M concentration, but was inefficient at low concentrations. Dibutyryl-cyclic AMP 2 mM stimulated total protein phosphorylation (+28%) but the secretory effect was subdued (+50%). The effects observed with cyclic GMP and its dibutyrylated derivative were insignificant. (2) In rat parotids, 10-5 M isoproterenol provoked a 10-fold increase in amylase output and exerted a slight (+15%) but significant effect on protein phosphorylation. These effects were reproduced by 0·5-2·0 mM dibutyryl-cyclic AMP. Dibutyryl-cyclic GMP at a high (5 mM) concentration allowed a slight increase (+86%) in enzyme secretion with no apparent stimulation of protein phosphorylation. (3) These results indicate a clear stimulation of protein phosphorylation when hormonal stimulus-secretion coupling was involved in the pancreas acinar cells and parotids. The derivatives of cyclic AMP partially mimicked these effects, and more clearly so in the parotids than in the pancreas. © 1975.