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Étude au microscope électronique de l'effet des rayons x sur l'évolution des structures nucléaires et cytoplasmiques au cours de la segmentation des oeufs de Pleurodeles waltlii

par Alexandre, Henri ;Gerin, Yves
Référence Experimental cell research, 65, 1, page (145-155)
Publication Publié, 1971

Article révisé par les pairs

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Résumé :

X irradiation of Pleurodeles embryos, at the beginning of cleavage, inhibits the lobulisation of the nuclei and the formation of small cytoplasmic invaginations which appear in the normal advanced blastulae. This inhibition seems to be closely linked to the inhibition of nuclear metabolism. An inhibition of the appearance of the outer granular constituent in the nucleoli would correspond to a decrease in rRNA synthesis. This inhibition is proportional to the X-ray dose. The lysis of the embryos is characterized by the existence of annulated lamellae either in or outside the nuclei and especially by the appearance of cytolysomes containing degenerated mitochondriae, vesicles of glycogen, lamellar structures. Finally, we have observed the presence of glycogen granules associated into chains in the intercellular spaces of both control and irradiated embryos. However, the irradiated embryos seem to be characterized by a higher content of glycogen; the inhibition of the utilisation of glycogen might explain this fact. © 1971 Academic Press Inc.