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DNA polymerase α, β and γ activities in rabbit spleen cell populations stimulated by various doses of concanavalin A

par Spadari, Silvio;Villani, Giuseppe ;Hardt, Nadine
Référence Experimental cell research, 113, 1, page (57-62)
Publication Publié, 1978

Article révisé par les pairs

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Résumé :

We have studied the correlation between the activities of DNA polymerase α, β and γ and the replication of DNA in spleen lymphocytes stimulated by suboptimal doses of ConA (0.5 and 3.5 μg/ ml) at which only initiation of DNA synthesis is observed, and by optimal ones (1 and 5 μg/ml) which permit both initiation of DNA synthesis and elongation of DNA chains in each lymphocytes subpopulation (cf ref. [19, 20]). Following stimulation of lymphocytes with both suboptimal and optimal doses of ConA, the activity of DNA-polymerase α rises within 24 h and parallels DNA synthesis rate; polymerase β shows no change and DNA polymerase γ activity decreases throughout the stimulation time as well as in unstimulated control cultures. Thus DNA polymerase α seems necessary during both initiation of DNA synthesis and elongation. The inhibition of elongation of DNA and therefore of cellular division and proliferation observed at suboptimal doses is not due to a lack of DNA polymerase activities. It is suggested that this inhibition could be due to lack in protease induction. © 1978.