par Moens, William ;Vokaer, Alain;Kram, Raphael
Référence Proceedings of the National Academy of Sciences of the United States of America, 72, 3, page (1063-1067)
Publication Publié, 1975
Article révisé par les pairs
Résumé : Mouse fibroblasts transformed by simian virus 40 (SV3T3 cells) are characterized by cyclic AMP and cyclic GMP levels, respectively, about half and twice those found in growing untransformed 3T3 cells. Density dependent inhibition of growth is correlated with reduced cyclic GMP concentrations in 3T3 and 4 different density restricted revertant lines derived from SV3T3. The levels of cyclic AMP are not increased at confluence. Upon serum restriction, serum dependent cell lines show a greater increase in intracellular cAMP than serum insensitive lines. Cyclic GMP levels are greatly reduced, even in serum insensitive density revertants, but not in SV3T3. Serum readdition to all serum dependent lines is followed by a rapid decrease in cyclic AMP and increase in cyclic GMP concentrations. The magnitude of these responses is decreased in SV3T3 and density revertants.