par Marcelis, Lionel 
;Surin, Mathieu;Lartia, R.;Moucheron, Cécile ;Defrancq, Eric;Kirsch-De Mesmaeker, Andrée
Référence European Journal of Inorganic Chemistry, 2014, 19, page (3016-3022)
Publication Publié, 2014
;Surin, Mathieu;Lartia, R.;Moucheron, Cécile ;Defrancq, Eric;Kirsch-De Mesmaeker, Andrée Référence European Journal of Inorganic Chemistry, 2014, 19, page (3016-3022)
Publication Publié, 2014
Article révisé par les pairs
| Résumé : | Under irradiation, Ru-TAP (TAP = 1,4,5,8-tetraazaphenanthrene) complexes tethered to oligonucleotides (Ru-ODN) hybridized to their complementary target sequences produce irreversible adducts with guanine (G) bases. The interesting feature is that the G bases that react in the double strand (DS) topology belong exclusively to the complementary strand; thus, no G base of the Ru-ODN strand participates in the production of these adducts. This highly specific photoreaction in the DS topology is demonstrated by gel electrophoresis analyses of different duplex sequences in which the positions of the G bases are varied. Atomistic molecular modelling simulations indicate that this specificity is due to important steric constraints for the intrastrand adduct formation in the duplex. Luminescence lifetime measurements furnish more detailed information on these different duplex systems. Under irradiation, a Ru-TAP (TAP = 1,4,5,8-tetraazaphenanthrene) complex linked to an oligonucleotide (violet) hybridized with its complementary sequence (green) is covalently anchored specifically to the G bases of the complementary sequence and not the G bases along its own sequence. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
