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Identification and characterization of α2-adrenergic receptors in human myometrium by [3H]rauwolscine binding

par Bottari, Serge P. ;Vokaer, Alain Patrick ;Kaivez, Etienne
Référence American journal of obstetrics and gynecology, 146, 6, page (639-643)
Publication Publié, 1983

Article révisé par les pairs

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Résumé :

Human myometrium contains α3-adrenergic receptors which can be identified by binding of the α2-adrenergic antagonist [3H]rauwolscine. Scatchard analysis of saturation binding data on myometrial membranes revealed that [3H]rauwolscine bound to a single class on noncooperative sites (262 ± 89 fmol/mg of membrane protein) with high affinity (i.e., with an equilibrium dissociation constant of 5.3 ± 2.2 nM). The α2-adrenergic nature of these sites was derived from the order of potencies and stereospecificity of α-adrenergic agonists and antagonists to compete with [3H]rauwolscine binding.