par Hens, Luc;Kirsch-Volders, Micheline 
;Arrighi, Frances;Susanne, Charles
Référence Human genetics, 53, 3, page (363-370)
Publication Publié, 1980
;Arrighi, Frances;Susanne, Charles Référence Human genetics, 53, 3, page (363-370)
Publication Publié, 1980
Article révisé par les pairs
| Résumé : | Trypsin-banded metaphase plates provided by one whole blood culture of a normal adult female were analyzed as to the chromosome distribution by measuring: distances between centromeres; angles formed between a centromere, the gravity center of the metaphase plane, and a second centromere; and the measured 'tendency to associate', as defined by Galperin. These data are correlated with Ag-NOR staining findings obtained from 72 cells from another culture of the same individual. In these cells, the chromosome pairs are identified using a simultaneous Ag-NOR staining and acridine orange banding technique. The silver precipitation is also correlated with the scored satellite associations in these cells. The results show a correlation between all concerned parameters, indicating that the nucleolar function of the human acrocentric chromosomes, as demonstrated by the silver precipitation technique, is probably one of the major determinants of the proximity of these chromosomes. There is a pronounced correlation of the Ag-NOR findings with those measured parameters which describe best the preferential small distances between chromosomes (angle analysis and 'tendency to associate' data). Moreover, the association patterns of the acrocentrics with small amounts of NOR provide some evidence for the interference of other determinants co-governing the position of the human D- and G-group chromosomes. |
