par Catarino, Ana A.I.;Cabral, Henrique H.N.;Peeters, Kris;Pernet, Philippe ;Punjabi, Usha;Dubois, Philippe
Référence Environmental toxicology and chemistry, 27, 7, page (1553-1560)
Publication Publié, 2008-07
Référence Environmental toxicology and chemistry, 27, 7, page (1553-1560)
Publication Publié, 2008-07
Article révisé par les pairs
Résumé : | The present study evaluated the effects of field metal contamination on sperm motility and the RNA/DNA ratio in echinoderms. Populations of Asterias rubens and Echinus acutus that occur naturally along a contamination gradient of sediments by cadmium, copper, lead, and zinc in a Norwegian fjord (the Sørfjord) were studied. Sperm motility, a measure of sperm quality, was quantified using a computer-assisted sperm analysis system. The RNA/DNA ratio, a measure of protein synthesis, was assessed by a one-dye (ethidium bromide)/one-enzyme (RNase), 96-well microplate fluorometric assay. Although both species accumulate metals at high concentrations, neither sperm motility parameters in A. rubens nor the RNA/DNA ratio in both species were affected. The Sørfjord is still one of the most metal-contaminated marine sites in Europe, but even so, populations of A. rubens and E. acutus are able to endure under these conditions. © 2008 SETAC. |