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Functional role of TTF-1 binding sites in bovine thyroglobulin promoter

par Javaux, F.;Bertaux, F.;Donda, Alena;Fancis-Lang, H.;Vassart, Gilbert ;Di Lauro, Roberto;Christophe, Daniel
Référence FEBS letters, 300, page (222-226)
Publication Publié, 1992

Article révisé par les pairs

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Résumé :

We have studied the binding of purified TTF-1 on the bovine thyroglobulin gene promoter. DNase I footprinting experiments revealed three binding sites which corresponded in location to the A, B and C sites found in the rat thyroglobulin promoter. Mutants in the A and C regions showing reduced binding of TTF-1, also exhibited largely decreased promoter activity in transient expression experiments in primary-cultured dog thyrocytes. Two mutants in the B site that exhibited a reduced capacity to bind TTF-1 also displayed a drastically affected transcriptional activity in transient assays. As in the rat, sites A and C only are critical for promoter activity, these results suggest that full occupancy of the B site is required for thyroglobulin promoter activity in the cow only.