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Loss of type IV collagen α5 and α6 chains in human invasive prostate carcinomas

par Dehan, Pierre ;Waltregny, David;Beschin, Alain ;Noël, Agnès;Castronovo, Vincent;Tryggvason, Karl;De Leval, Jean;Foidart, Jean-Michel
Référence The American journal of pathology, 151, 4, page (1097-1104)
Publication Publié, 1997-10

Article révisé par les pairs

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Résumé :

Type IV collagen, a major component of basement membranes, is organized in a network responsible for the mechanical resistance of the basement membranes. It also plays a key role in epithelial cell adhesion to basement membranes. This study was designed to investigate the distribution of type IV collagen α-chains in normal, preneoplastic, and malignant prostate basement membranes. For this purpose, immunohistochemistry using specific antibodies raised against the different α-chains of type IV collagen was performed in eight normal samples, six prostatic intraepithelial neoplasia, and 20 malignant lesions of the prostate. Our results demonstrate the presence of the 'novel' α5 (IV) and α6 (IV) chains along with the 'classical' α1 (IV)/α2 (IV) chains in the basement membrane of the normal prostate gland. The α3 (IV) chain was never detected in any prostate specimen. Prostatic intraepithelial neoplasia showed a similar immunostaining pattern to that found in normal glands. In cancer gland basement membranes, we demonstrate for the first time a specific loss of the α5 (IV) and α6 (IV) chains, whereas the classical α1 (IV) and α2 (IV) chains were consistently exhibited. Additionally, type VII collagen colocalized with α5 (IV) collagen chain, and these two proteins, which were always observed in normal and prostatic intraepithelial neoplasia gland basement membranes, were lost in invasive carcinoma basement membranes. This observation raises questions about the possible association or cooperation between α5 (IV)/α6 (IV) chains and anchoring fibrils in prostate glands basement membrane.