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The use of l-[35S]methionine and l-[75Se]selenomethionine for double-label autoradiography of complex protein patterns on two-dimensional polyacrylamide gels: A drastic shortening of the exposure time

par Lecocq, Raymond ;Hepburn, Anne ;Lamy, Françoise
Référence Analytical biochemistry, 127, 2, page (293-299)
Publication Publié, 1982-12

Article révisé par les pairs

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Résumé :

A method of autoradiography that allows discrimination between 35S- and 75Se-labeled material on polyacrylamide gels is described. It involves the incorporation of l-[35S]methionine and l-[75Se]selenomethionine into proteins. The autoradiographic exposure times are decreased at least sixfold with regard to the previously described techniques using 3H- and 14C-labeled proteins. © 1982.