• Citer

Low mutarotase activity in normal and tumoral pancreatic islet cells

par Rasschaert, Joanne ;Zahner, Dagmar ;Malaisse, Willy
Référence Medical science research, 15, page (621-622)
Publication Publié, 1987

Article révisé par les pairs

• Détails
• Contenu

Résumé :

The metabolism of D-glucose displays anomeric specificity in several but not all tissues. It is not known whether such a difference is related to the level of mutarotase activity in these distinct cell types. Mutarotase activity was measured, therefore, in rat kidney, liver, pancreas, lens, pancreatic islet and tumoral islet cell (RINm5F line) homogenates. Relative to tissue wet weight, the highest activity was recorded in liver and kidney, in which tissues it was at least 10 times greater than lens or pancreas. In pancreatic islets or tumoral islet cells, mutarotase activity was close to the limit of detection. There was no parallelism between mutarotase activity and the presence, or absence, of anomeric specificity for D-glucose metabolism in intact cells. The fact that the highest enzymic activity was found in the 2 major organs with detoxicating function raises the teleological point that mutarotase may play a role in the clearance of foreign and undesirable sugars and sugar derivatives.