Detection of group a streptococcal pharyngitis by quantitative PCR.

Dunne, Eileen M; Marshall, Julia L; Baker, Ciara A; Manning, Jayne; Gonis, Gena; Danchin, Margaret H; Smeesters, Pierre; Satzke, Catherine; Steer, Andrew C

doi:doi/10.1186/1471-2334-13-312

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Detection of group a streptococcal pharyngitis by quantitative PCR.

par Dunne, Eileen M ;Marshall, Julia L ;Baker, Ciara A ;Manning, Jayne ;Gonis, Gena ;Danchin, Margaret H ;Smeesters, Pierre

;Satzke, Catherine ;Steer, Andrew C
Référence BMC infectious diseases, 13, page (312)
Publication Publié, 2013

Article révisé par les pairs

Résumé :

Group A streptococcus (GAS) is the most common bacterial cause of sore throat. School-age children bear the highest burden of GAS pharyngitis. Accurate diagnosis is difficult: the majority of sore throats are viral in origin, culture-based identification of GAS requires 24-48 hours, and up to 15% of children are asymptomatic throat carriers of GAS. The aim of this study was to develop a quantitative polymerase chain reaction (qPCR) assay for detecting GAS pharyngitis and assess its suitability for clinical diagnosis.

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