par Dieye, Tandakha;Van Vooren, Jean-Paul 
;Delforge, Marie-Luce ;Liesnard, Corinne ;Devleeschouwer, Michel ;Farber, Claire
Référence Biomedicine & pharmacotherapy, 54, 1, page (16-20)
Publication Publié, 2000-02
;Delforge, Marie-Luce ;Liesnard, Corinne ;Devleeschouwer, Michel ;Farber, Claire Référence Biomedicine & pharmacotherapy, 54, 1, page (16-20)
Publication Publié, 2000-02
Article révisé par les pairs
| Résumé : | Increased programmed cell death (PCD) or apoptosis has been detected in the T cells of HIV-infected subjects; it is held partially responsible for the continuous loss of CD4+ T cells during the natural course of HIV infection. Highly active antiretroviral therapy (HAART) decreases the viral load and leads to an increase of CD4+ count in vivo. In this study we evaluated PCD in total peripheral blood mononuclear cells, CD8+ and CD4+ lymphocytes before and four weeks after initiation of HAART. Seven HIV-1-infected patients were investigated. Viral load was assessed by RT-polymerase chain reaction and PCD by flow cytometry using apoptosis by 7 amino actinomycin D (7AAD) and propidium iodide (PI). After four weeks of HAART, CD4+ T and CD8+ T cell levels were stable, and plasma HIV-RNA copies were significantly decreased. In four of the patients (4/7), HIV-RNA levels were reduced to undetectable levels (fewer than 400 copies per milliliter). A statistically significant reduction of apoptosis among CD4+ cells was observed (P < 0.03), though neither in the CD8+ T cell population nor in peripheral blood mononuclear cells (PBMCS). These results demonstrate the beneficial effect of HAART on apoptosis of CD4+ cells in the early treatment stage. |
