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A PCR based DNA hybridisation capture system for the detection of human cytomegalovirus. A comparative study with other identification methods

par Mansy, Frédéric ;Brancart, Francoise ;Liesnard, Corinne ;Bollen, Alex ;Godfroid, Edmond
Référence Journal of virological methods, 80, 2, page (113-122)
Publication Publié, 1999-07

Article révisé par les pairs

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Résumé :

A simple, sensitive and specific colourimetric hybridisation method for the detection of HCMV DNA in clinical specimens is described. This method combines a PCR assay with a sensitive sandwich hybridisation assay. It relies on the use of a specific capture probe linked covalently to polystyrene microplates and a specific polybiotinylated detection probe. Amplified DNA fragments, sandwiched between these two probes, are detected by an enzymatic colour reaction. This PCR-based colourimetric hybridisation method was compared with other known HCMV detection methods. Clinical specimens (n=145, corresponding to 106 patients) were tested by both a nested PCR assay and this colourimetric hybridisation method; and by either the culture method or the pp65 antigenaemia test depending on the type of sample used. The results showed that the PCR-based hybridisation method has a specificity similar to tissue culture, known as the conventional gold standard method, and could be used for the examination of the clinical specimens. Copyright (C) 1999 Elsevier Science B.V.