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Different chromatographic behaviours on blue Sepharose CL-6B of free and estradiol complexed forms of the estrogen receptor from DMBA-induced rat mammary tumors

par Tenenbaum, Anita ;Leclercq, Guy
Référence Journal of Steroid Biochemistry, 13, 7, page (829-833)
Publication Publié, 1980

Article révisé par les pairs

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Résumé :

Free and estradiol-complexed forms of the cytoplasmic estrogen receptor from DMBA-induced rat mammary tumors display a different chromatographic behaviour on Blue Sepharose CL-6B. At 4°C, the complexed form had a low affinity for the Blue Sepharose matrix since it was washed from the column with the non-binding proteins. On the contrary, the free receptor appeared tightly adsorbed on the Blue Sepharose since it was only recovered by eluting the column with a 0.5 mg/ml solution of Cibacron Blue. Additional studies showed that adsorbed receptor could also be extracted with 10-8 M [3H]-estradiol or [3H]-diethylstilbestrol at 18°C. It is proposed that this difference in chromatographic behaviour between the free and complexed form of the receptor could be of hydrophobic nature.