par Cornet, Bernard 
;Decroly, Etienne ;Thines-Sempoux, D;Ruysschaert, Jean Marie ;Vandenbranden, Michel
Référence AIDS research and human retroviruses, 8, 10, page (1823-1831)
Publication Publié, 1992-10
;Decroly, Etienne ;Thines-Sempoux, D;Ruysschaert, Jean Marie ;Vandenbranden, Michel Référence AIDS research and human retroviruses, 8, 10, page (1823-1831)
Publication Publié, 1992-10
Article révisé par les pairs
| Résumé : | Human immunodeficiency virus (HIV) membrane has been reconstituted from the recombinant envelope glycoprotein precursor (gp160) by a detergent dialysis technique. Electron microscopy shows that gp160-virosomes are spherical vesicles with a mean diameter identical to that of viral particles. Enzyme-linked immunosorbent assay and immunogold labeling demonstrate efficient association of gp160 with lipid vesicles and proteolysis treatment reveals an asymmetric insertion with about 90% of glycoproteins having their gp120-moiety pointing outside. Glycoproteins are organized as dimers and tetramers and gp160 retains its ability to specifically bind CD4 receptor after reconstitution into virosome. |
