par Gomis, Ramon;Sener, Abdullah 
;Malaisse Lagae, Francine ;Malaisse, Willy
Référence Biochimica et biophysica acta (G). General subjects, 760, 3, page (384-388)
Publication Publié, 1983
;Malaisse Lagae, Francine ;Malaisse, Willy Référence Biochimica et biophysica acta (G). General subjects, 760, 3, page (384-388)
Publication Publié, 1983
Article révisé par les pairs
| Résumé : | Pancreatic islet homogenates catalyze, in a Ca2-dependent fashion, the incorporation of [2,5-3H]histamine, [1,4-14C]putrescine, [1,2-3H]agmatine, [14C]methylamine and L-[U-14C]lysine in N,N-dimethylcasein. Using [2,5-3H]histamine as the amine donor the K(m) for Ca2+ and histamine amounts to 90 μM and 0.7 mM, respectively. The incorporation of [2,5-3H]histamine into N,N-dimethylcasein is inhibited by monodansylcadaverine, N-p-tosyl glycine, bacitracin and methylamine, the relative extent of inhibition depending on the respective concentrations of Ca2+, inhibitor and amine donor. Bacitracin and methylamine, but not N-p-tosyl glycine, cause a dose-related inhibition of glucose-stimulated insulin release. It is concluded that, in pancreatic islets, the Ca2+-responsive transglutaminase activity plays a critical role in the process of glucose-induced insulin release. |
