par Sener, Abdullah 
;Ramirez, Remedios;Malaisse, Willy
Référence Biochemical medicine and metabolic biology, 47, 2, page (116-123)
Publication Publié, 1992
;Ramirez, Remedios;Malaisse, Willy Référence Biochemical medicine and metabolic biology, 47, 2, page (116-123)
Publication Publié, 1992
Article révisé par les pairs
| Résumé : | A radioisotopic procedure for the assay of myo-inositol is presented. It is based on the generation of NADH from NAD+ in the reaction catalyzed by myo-inositol dehydrogenase and the subsequent NADH-dependent conversion of 2- [U-14C]ketoglutarate to 14C-labeled L-glutamate in the reaction catalyzed by glutamate dehydrogenase. This method was applied to the measurement of myo-inositol in rat pancreatic islets. The myo-inositol islet content was decreased when the animals were fed a diet deprived of myo-inositol. When incubated in the absence of exogenous D-glucose, pancreatic islets, like parotid cells, released myo-inositol in the incubation medium. Over 90 min of incubation, a rise in extracellular D-glucose concentration increased the myo-inositol islet content, which was decreased, however, after incubation in the presence of carbamylcholine. These findings indicate that the myo- inositol content of islets is affected by nutritional and other environmental factors. |
