par Sener, Abdullah 
;Malaisse Lagae, Francine ;Gervy Decoster, Christine ;Malaisse, Willy
Référence Cell biochemistry and function, 11, 2, page (145-151)
Publication Publié, 1993-06
;Malaisse Lagae, Francine ;Gervy Decoster, Christine ;Malaisse, Willy Référence Cell biochemistry and function, 11, 2, page (145-151)
Publication Publié, 1993-06
Article révisé par les pairs
| Résumé : | In thyroidectomized rats, the activity of FAD-linked glycerophosphate dehydrogenase was severely diminished in liver homogenates but not affected significantly in pancreatic islet homogenates, whilst the activity of 2-ketoglutarate dehydrogenase was decreased modestly in both liver and islet homogenates. Likewise, in intact islets of thyroidectomized rats, the generation of3HOH from [2-3H]glycerol was not decreased, and the ratio between oxidative and total glycolysis not significantly lower than in islets from sham-operated rats, at least in the presence of a high concentration of D-glucose. Nevertheless impaired oxidation of both D-[3,4-14C]glucose and D-[6-14C]glucose was observed in islets of thyroidectomized rats, the relative magnitude of such a decrease being more pronounced at a low than at a high D-glucose concentration. Such metabolic anomalies coincided with a lower level of plasma insulin and a decreased output of insulin by islets incubated at low (2·8 mM), but not higher, concentrations of D-glucose. It is concluded that hypothyroidism does not mimic the deficiency in islet FAD-linked glycerophosphate dehydrogenase activity found in rats with inherited or acquired non-insulin-dependent diabetes. |
